Liverpool striker Daniel Sturridge has been left out of the England squad for the Euro 2016 qualifiers against Estonia and Lithuania.
Tottenham midfielder Dele Alli, 19, and Reds forward Danny Ings, 23, have received their first senior call-ups, having represented England Under-21s.
Sturridge, who has just recovered from a hip injury, scored twice in Saturday’s 3-2 win over Aston Villa.
Reds boss Brendan Rodgers said he could not manage both England games, reports the BBC.
Aston Villa midfielder Jack Grealish, who opted to play for England rather than the Republic of Ireland, is awaiting international clearance.
England has already qualified for next year’s European Championship in France.
In their final two group games they face Estonia at Wembley on October 9 and then Lithuania away three days later.
Alli signed for Spurs from MK Dons in February, ending the season on loan with the League One side.
In August he made his Spurs debut – his first of eight appearances so far – and last month won his first England Under-21 cap.
Ings, who was at the European Championship with England Under-21s this summer, joined Liverpool from Burnley in the summer and has scored twice for the Reds.
Also returning to the squad are Southampton defender Ryan Bertrand, Manchester United’s Phil Jones and Liverpool midfielder Adam Lallana, who missed September’s matches against San Marino and Switzerland with injury.
Bertrand will replace Manchester United left-back Luke Shaw, who is expected to be out for six months after breaking his leg in the Champions League defeat by PSV Eindhoven on September 15.
FULL SQUAD
Goalkeepers: Jack Butland (Stoke City), Joe Hart (Manchester City), Tom Heaton (Burnley)
Defenders:Ryan Bertrand (Southampton), Gary Cahill (Chelsea), Nathaniel Clyne (Liverpool), Kieran Gibbs (Arsenal), Phil Jagielka (Everton), Phil Jones (Manchester United), Chris Smalling (Manchester United), John Stones (Everton)
Midfielders: Dele Alli (Tottenham Hotspur), Ross Barkley (Everton), Michael Carrick (Manchester United), Adam Lallana (Liverpool), James Milner (Liverpool), Alex Oxlade-Chamberlain (Arsenal), Jonjo Shelvey (Swansea City), Raheem Sterling (Manchester City)
Forwards: Danny Ings (Liverpool), Harry Kane (Tottenham Hotspur), Wayne Rooney (Manchester United), Jamie Vardy (Leicester City), Theo Walcott (Arsenal)