Carlin Q. Williams, 39, through his attorney in early May claimed he was Prince's son, which if true would have entitled him to at least part of Prince's estimated $300 million estate. A second source also confirmed to NBC News that the test result was negative.
Williams' mother, Marsha Henson, wrote in a court affidavit that she had unprotected sex with Prince, whose full name is Prince Rogers Nelson, in a Missouri hotel room in 1976.
Henson told The Associated Press in a brief interview that she does not believe the reported test results.
"I don't believe that. I think they're positive," she said.